ABSTRACT
This study was conducted on cucumber seeds variety of [[DAMEX, Fl line -105] and barley seeds variety of [Aruge, lineS-R]. Effort was made to compare germination and growth of cucumber and barley seeds under in vitro condition by using of both polyacryiamide [PCA] and Czapek dox ager media. Both media were supplied with growth regulators Gibberellic acid [Gas] at concentrations 4ppm, IGppm and Indol-3-buteric acid [3-IBA] at concentrations 4ppm and 16 ppm respectively. After weeks of growth and germination, seeds were transferred to soil pots for adaption. Important seedling characters like total seedling length, root length and shoot length were recorded also DNA amount estimated. The result shown that, no significant difference was observed in total seedling length between both polyacrylmide and Czapek dox ager media also estimation of DNA amount does not reveal to any significant differences, barley seedling that grown in Czapek dox ager media shown an increase in their shoot lenght compared with barley seedling grown in polyacryiamide media. Root length increased significantly in barley seeds grown in polyacryiamide media only. It can be noticed that, cucumber seedlings grown on polyacriyamide media showed an increase in their shoot length while cucumber seedling that grown on czapek dox ager media showed an increase in their root length. Growth regulators [GAs and 3-IBA] increased root length of both cucumber and barley seedling. Also they increased root length of barley seedling that grown in czapek dox ager media